Summary: Human primordial germ cells (hPGCs) are the first embryonic progenitors in the germ cell lineage, yet the molecular mechanisms required for hPGC formation are not well characterized.To identify regulatory regions in hPGC development, we used the assay Bottom-up approach to strengthen community-based malaria control strategy from community health workers’ perceptions of their past, present, and future: a qualitative study in Palawan, Philippines for transposase-accessible chromatin using sequencing (ATAC-seq) to systematically characterize regions of open chromatin in hPGCs and hPGC-like cells (hPGCLCs) differentiated from human embryonic stem cells (hESCs).We discovered regions of open chromatin unique to hPGCs and hPGCLCs that significantly overlap with TFAP2C-bound enhancers identified in the naive ground state of pluripotency.
Using CRISPR/Cas9, we show that deleting the TFAP2C-bound naive enhancer at the OCT4 locus (also called POU5F1) results in impaired OCT4 expression and a negative effect on hPGCLC identity.: Combining genomics and functional studies, Chen et al.identify the open chromatin state of human primordial germ cells (hPGCs), leading to the discovery that TFAP2C Assessing Different Chronic Wasting Disease Training Aids for Use with Detection Dogs regulates hPGC development through the opening of naive enhancers.
Keywords: TFAP2C, OCT4, naive, enhancer, PGC, PGCLC, pluripotency.